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endoenzyme n.【生物學】(胞)內酶。

endogamic

This time , using cdna of zmcdc5 as template , we amplify a sequence by means of pcr technology . and then , using restrict endoenzyme and ligase , we conjunct the 0 . 8kb length dna sequence in a expression vector , pet - 30a . after induction , expression and purification , we obtained a 35 . 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ) . with the purified protein , we got its antibody and testified the antibody by means of western blotting and dot blotting 本實驗是以zmcdc5的cdna為模板,使用pcr獲得基因片段,再通過酶切連接,將得到的0 . 8kb的基因片段構建于pet - 30a表達載體上,經過誘導表達和純化,獲得zmcdc5的融合蛋白,其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基

Constructing human colorectal cancer cell line with stably down - regulated grp94 ( 1 ) the plasmid prc / rsv - ribol that contains specific grp94 - targeting ribozyme and the control plasmid prc / rsv were miniprepared , respectively , cleaved by endoenzyme pvuii 穩定下調grp94的人大腸癌細胞克隆株的構建( 1 )分別對含有特異性打靶grp94核酶的質粒prc rsv - ribo1和對照組質粒prc rsv進行小量提取、 pvu酶切鑒定。