E - elute 中文

elute vt.【化學】洗提。elution n.

elutriate
During the course of purification , the method of orthophenanthroline staining is used to detect the ferritin . 30 \| il of the fractions eluted from the aca ^ gel column were pulled into the cells of the minim incubation plate . then sopil 20 % dithionite and 40 \| il 0 . 1 % orthophenanthroline solution were pulled into respectively 在酶標板小孔中加入30 l待測液，加入30 l20的連二亞硫酸鈉溶液還原后，再加入40 l0 . 1鄰菲咯啉溶液，根據有無桔紅色顏色產生確定是否含鐵蛋白。
After bacterium cells were treated by formaldehyde , surface proteins of gram - negative bacterium e . coli k12 were eluted little , but it had no influence on adsorption of snake venom proteins to bacterium cells . treatment by formaldehyde had little influence on snake venom absorption to and deabsorption from gram - positive bacterium streptococcus mutans , but absorbance of interaction protein with molecular weight of 47kda only adsorbed by streptococcus aureus was greatly decreased 結果表明，滅活后的革蘭氏陰性菌( e . colik12 )再與蛇毒相互作用時，洗脫下來的蛋白中菌體蛋白含量明顯減少，但不影響對作用蛋白的吸附；甲醛滅活對革蘭氏陽性菌( s . mutans和s . aureus )影響不大，只有s . aureus吸附蛋白中47kda的蛋白吸附量減少，不會影響作用蛋白中其它蛋白的吸附。
The powder was then applied to a silica gel column chromatography charged with 80 % acetone in water and eluted with the same solvent . after concentrating and drying , relatively pure fr - 008 was obtained . hplc assay a comparison between antibiotic fr - 008 and candicidin has been made by studying the hplc separation profiles of antibiotic fr - 008 , candicidin and a mixture of both 對鏈霉菌fr - 008和灰色鏈霉菌imru3570的rflp分析發現，它們的染色體具有很高同源性； pfge的比較研究發現，這兩個菌株的明顯區別是鏈霉菌fr - 008攜帶有兩個線性質粒，而在灰色鏈霉菌imru3570中則沒有這兩個線性質粒。
At first , 1 . 67 u g per well mcab all was coated on three wells of a plate , and then 1 . 5 x 1011 phage virion was diluted and added , after incubating with the target , wash away unbound phage by tbst ( 0 . 1 % tween - 20 ) , the bound phage was eluted with ph 2 . 2 tris - gly buffer and amplified , the specially bound phage was enriched by taking through addition binding / amplification cycles . ln the following cycles , the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all , collecting and titering the washing phage of last time and output phage in each round , the selective ratio and the false positive rate of each round were worked out , the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective . after 4 rounds of panning , 11 phage clones were selected after competitive - ellsa , the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced , a clear consensus binding sequence emerged 在本實驗中，利用隨機12肽庫對抗豬瘟病毒( classicalswinefeverviruscsfv )糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以后，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以后經過dnastar軟件分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2 , then phage was added to the wells . after incubation , the wells were washed vigorously with tbst to remove nonbinding phage . phage bound to the antibody were eluted with 0 . 2mol / l glycine - hcl ( ph2 . 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9 . 1 ) 以抗h3n2流感病毒的多克隆抗體( 100ng l )包被酶標板，加入制備好的肽庫，用tbst洗去非特異結合的噬菌體，加0 . 2mol l甘氨酸-鹽酸( ph2 . 2 ) ，室溫放置10min以洗脫特異結合的噬菌體， 2mol ltris - hcl ( ph9 . 1 )中和后，取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定，其余噬菌體擴增后用于下一輪篩選，共重復3輪淘洗。
Finally , avirulent and virulent plant , bacteria of pseudomonas solanaceanim was investigated by chromatography . the result showed that there are two peaks when both bacteria was eluted , but their peak area ratio was quite different . when virulent bacteria was subject to chromatography after vibration by ultrasonic wave , both chromatographial behaviors of both avirulent and virulent pseudomonas solanaceanim were same basically , which showed a equilibrial peak and a eluting one 最后，表征了植物病原菌? ?青枯菌的強、弱致病菌株，結果顯示強、弱菌都有兩個洗脫峰，但峰面積比例有明顯的差異，強弱致病菌株經振蕩后，其色譜行為基本相似，表現為一個穿透峰和一個洗脫峰。
The ps ii native fractions ( 20 % and 30 % ) were loaded onto a deae column . the fraction eluted with 150 mm nacl was presented dcip reduction activity and was highly depleted in chi c and xanthophylls , and as such could be considered a ps ii core complex 對于有dcip光還原活性的20和30層帶的復合物，進一步deae離子交換層析純化。 150mmnacl洗脫純化后的樣品經過熒光激發光譜測定發現，已經去除了葉綠素c和墨角藻黃素，并且仍然具有dcip的光活性，分析是ps核心復合物。
Method was investigated to extract multiple proteins from complex system including water - soluble fraction of egg yolk and agkistrodon acutus venom by bacterial specific absorption . immunoglobulin yolk ( igy ) were absorbed directly from water - soluble fraction by streptococcus mutans , then eluted and further purified by columns of thiophilic gel . based on above experiments of antibody extraction , bacterium cells were applied further to extract interactive proteins from crude venom of agkistrodon acutus 首先，利用變異鏈球菌( s . mutans )與其特異性抗體的吸附作用從水溶性蛋白組分中分離出卵黃抗體( igy ) ，并將菌體上洗脫下來的igy用嗜硫色譜分離純化，得到高純度的細菌特異性抗體。
New york ( reuters health ) , feb 13 - ahead of its scheduled publication date of march 8 , the new england journal of medicine has released fie original articles and two editorials regarding the risk and benefits of drug - eluting stents for treatment of coronary artery stenosis 紐約（路特健康） 2月13日- - - 《新英格蘭醫學》雜志比計劃出版日期3月8日提前報道了關于使用藥物洗脫支架治療冠狀動脈狹窄好壞的5篇研究原文和2篇社論。
Add 1ml of 50 % ni - nta slurry to 4ml lysate and mix gently and load the mixture into an empty column . then wash and elute the column with different buffer in order to purify the target fusion protein with the interaction of its 6 x his - tag and ni - nta matrices Rt一pcr擴增片斷克隆于puc18質粒進行序列鑒定，結果顯示所擴增cdna片斷長13sbp ，序列分析與genbank中報道的編碼hpd一3成熟膚的cdna序列完全一致。
Background : estradiol promotes rapid re - endothelialization of coronary stents in animal models , but it is not known whether combining this drug with rapamycin represents an improed drug - eluting stent technology in terms of reduced lumen renarrowing 背景：動物實驗中，雌二醇可以促進冠脈支架的快速再內皮化，但是聯合應用雌二醇和雷帕霉素的藥物洗脫支架是否在減少管腔再狹窄方面更加有效，尚不得而知。
This material was loaded onto a silica gel column and the column was eluted with chloroform several times until the fraction of interest was collected . finally we obtained some light yellow green ointment liquid f i 采用硅膠作干層析柱填充物，用氯仿作展開劑和洗脫液，反復硅膠層析，對柄海鞘鞘囊氯仿浸提物進行分離、提取與純化，最后得到一淺黃綠色的油狀液體fraction (以下均稱f ) 。
New evidence is emerging to contradict earlier claims that drug - eluting stents cause increased risks for patients , although nice ' s recent preliminary guidance was against their use in coronary artery disease 藥物洗脫支架早前曾有藥物洗脫支架會導致病人風險增加的說法， nice近期發布的初步指南也反對在冠狀動脈疾病中使用藥物洗脫支架，但已有新證據結論與此相反。
Interest in this issue is high , because the multiple trials that hae ealuated the safety of drug - eluting stents compared with bare - metal stents for treatment of coronary artery stenosis hae yielded conflicting results 人們對該論點的興致非常高漲，因為評價用于治療冠狀動脈狹窄的裸露金屬支架和藥物洗脫支架安全性的反復實驗產生了相沖突的結論。
Amplified in e . coli xi - blue , the eluted phage in the third rounds was poured onto lb / iptg / xgal plate . we selected randomly 18 clones and amplified them , then confirmed positive clones by elisa 經雙抗體(流感病毒的多抗和辣根過氧化物酶標記m13噬菌體抗體)夾心elisa鑒定的陽性克隆有12個，分別將其純化、并進行dna序列分析。
Eeryone seems to agree on the necessity of more research on the risks and benefits of drug - eluting stents , patients for whom such stents are appropriate , and optimal duration of antiplatelet therapy 現在每人看來都認為有必要對藥物洗脫支架的好壞作更步的研究；一些病人適合用這樣的支架，并且抗血小板治療需要延長合適的時間。
New york ( reuters health ) , no 21 - polymer - based paclitaxel - eluting stents appear to be superior to non - polymer paclitaxel stents in the treatment of coronary lesions , german researchers report 紐約（路透社健康新聞） 11月21日：德國研究者報道聚合物基紫杉醇支架在治療冠脈病變時比非聚合物的紫杉醇支架顯得更有優越性。
Objective this study was designed to investigate the incidence of acute or sub - acute thrombosis following implantation of drug - eluting stents ( des ) and to compare it with bare metal stents to evaluate the des safety 摘要目的調查國內藥物洗脫支架的急性或亞急性血栓發生率，與金屬裸支架相比，評價其安全性。
The amplified phage be used repeat the selection . the selection was repeat three times . the titter of phage indicated that the eluted phage enriched with the rounds increasing 山西醫科大學碩士學位論文第三次淘洗的噬菌體感染大腸桿菌xli一blue ，鋪制平板，隨機挑取18個分離良好的噬斑，制備噬菌體原種。

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elute

elute vt.【化學】洗提。elution n.

elutriate