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cotyledon n.【植,解】絨毛葉,子葉。-donous, -dona...

cotyloid

Cotyledon without petiole showed a significantly lower frequency of shoot regeneration ( 16 . 7 % ) compared with that of entire cotyledon ( 33 . 8 % ) , but the average number of shoots per explant ( 4 . 2 ) was more than that of entire cotyledon ( 2 . 5 ) . no shoot was obtained when the petiole alone was cultured . the frequency of shoot regeneration ( 8 . 3 % , 12 . 5 % ) and average number of shoots per explant ( 2 . 3 , 1 . 7 ) from the upper section of lamina or one side of lamina was lower than those of entire cotyledon 另一方面,以“種都一號”兒菜的不同子葉部位作為外植體所進行的研究結果表明,不帶子葉柄的完整葉片其芽再生率( 16 . 70 )顯著低于帶子葉柄的完整子葉( 33 . 8 ) ,但平均再生芽數,前者( 4 . 2 )卻高于后者( 2 . 5 ) ;子葉柄不能進行芽的分化;不帶子葉柄的子葉尖端和子葉一側,其芽再生率(分別為12 . 5 、 8 . 30 )及再生芽數(分別為2 . 3 、 1 . 7 )也明顯低于帶子葉柄的完整子葉。

The results of variance analysis showed that there was no significant difference in the same organ of soybean varieties , but the difference was highly significant in different organs of same variety . the content of isoflavones in organs on the ground was much more than that of underground at the stage of coming out , and isoflavones in cotyledon was much more than in other tissues in the stage of seedling 營養生長期異黃酮的分布存在組織特異性而無種屬特異性,各組織間的差異達到極顯著水平:在大豆出苗期,地上部分的異黃酮含量遠高于地下部分;幼苗期子葉中異黃酮遠高于其它部分;壯苗期和盛花期,葉片中異黃酮的含量遠高于別的組織。

In this paper , genetic transformation systems by agrobacterhim rhizogems was established and optimized and high quality regenerating system was selected . the products of indigotin and indirubin in regenerating plantlets had been detected by hplc . the main studies and results were reported as follows : 1 establishment and selection of hairy root clones taken cotyledons of autotetraploid indigoblue woad as explants and hairy roots induced successfully from them by agrobaterium rhizogenes strains a4 and r1000 本文利用發根農桿菌rit - dna建立并優化了四倍體菘藍的遺傳轉化體系,并對再生植株的主要代謝產物靛藍、靛玉紅進行了含量檢測,篩選了優良無性系,也為實現外源抗蟲、抗病等基因的導入奠定了基礎。

4 . after having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time , incubation time and co - culture time , we set up the system of high frequency transformation of tomato cotyledons . then hbmp - 3m gene was transferred into tomato via agrobacterium - mqdiated transformation , and the resistant plants to hyg were obtained . by pcr analysis on part of the putative transformants , we identified that hbmp - 3m gene had been integrated into the genome of part of tomato plants . 5 . transferred hbmp - 3 gene into tobacco via agrobacterium - mediated transformation and obtained the resistant plants to hyg . trans genie tobacco plants were confirmed by instantaneous expression of gus gene in calli detection , growth and bio - morphology analysis , hyg - resistant experiment and pcr analysis 通過pcr檢測證實部分番茄抗性植株中已導入hbmp - 3m基因;人骨形成蛋白一3成熟膚基因和全長基因分別轉化番茄和煙草的研究5 .通過農桿菌介導法將hbmp一3全長基因導入煙草,并且獲得了hyg抗性植株,通過gus基因瞬時表達檢測、轉化植株的生長情況及形態學分析、 hyg抗性鑒定及pc尺檢測,證明目的基因己經整合到煙草基因組中。

S . regeneration system of mustard hypocotyls and cotyledons of 4 days and leaves of 25 days were used as transformation materials , and ms as basic medium supplying different ba ^ naa and kt concentration , we got the optimum explant adventitious bud inducing medium ( ms + 6 - ba3 . 0mg / l + naa0 . 2 mg / l ) . among the three kinds of mustard explants . the cotyledons showed the highest adventitious bud regeneration capacity . and the regeneration capacity of leaf explantswas weaker . and the weakest of hypocotyls 芥菜高頻再生體系的建立以4天左右的葉用和莖用芥菜子葉、下胚軸和25天苗齡的芥菜無菌苗的葉片為外植體,以ms為基本培養基,通過加入不同濃度和不同組合的ba 、 naa 、 kt ,篩選到了外植體不定芽分化的最適培養基( ms + ba3 . 0mg / l + naa0 . 1mg / l )及生根的最適培養基( ms + naa0 . 2mg / l ) 。

The genotype is a main factor in the genetic transformation via agrobacteriwn - mediated . the results of orthogonal experiments of the factors which affect mainly the transformation illuminates that the erniuxin genotype + bacterium 15 x + explant + dip - dye is the best one . the cocultivation time is 28 days . the experiment shows that : the erniuxin cotyledon regeneration frequency is very high , and that the dongnong901 hypocotyl regeneration frequency is very high 確立了農桿菌介導轉化過程中基因型是影響轉化率的主要因素。通過極差分析,基因型為“二牛心” ,菌液濃度15倍的,外植體為子葉,感染方法為浸泡法是最佳的組合。農桿菌與外植體共培養的時間為28h 。

There are also different in inducing cluster buds from callus of clone gingkgo and its other tissues and organs , some callus can induce cluster buds , but others can not . differentiation rate from the callus of cotyledon can be up to 36 % and the number of cluster buds is positive related to the times of subculturing 但是不同器官誘導的愈傷組織對于叢生芽的誘導也是不同的,子葉誘導的愈傷組織的分化率較高,有36左右,并且繼代次數的增加也能夠增加分化的幾率

Cotyledon and hypocotyl ' s rate and quamity are the most among these explams , and callus can be obtained in 10 days by cotyledon and hypocotyl . reversely it is difficult to indue callus with root , and the callus from root is lnde and easy to become browning . the calius obtained from leaf grows very slow and does not become browning uniill in 2 or 3 months 銀杏的不同器官和組織都能夠誘導出愈傷組織來,其中,子葉和胚軸10d左右全部愈傷化,誘導速度和誘導率均最高,根則很難誘導,愈傷組織很少,褐化很快;葉片誘導的愈傷組織,生長慢,褐化也慢,在培養基上保持兩三個月而不褐化;胚乳的誘導時間也較長,需要30d左右。

Gibberillin content of shoot apices in plant was 5 times higher than that of control after ga treatment . cold treatment promoted synthesis of endogenous ga . the content of ga in seedlings at cotyledon stage , shoot apices and leaves of seedlings were 4 times , 2 . 5 times and 170 % as much as control after cold treatment 外源赤霉素處理使植株莖尖的赤霉素含量達到對照的5倍多;低溫處理促進內源赤霉素含量的增加,萌動種子和幼苗低溫處理后子葉期幼苗和幼苗的莖尖、葉片分別比對照和低溫處理前增加了3倍、 1 . 5倍和70 。

Soybean isoflavones distribution was investigated in vegetative growth period ( four stages before seeding ) . the isoflavones in soybean seeds transferred from cotyledon to other organs and isoflavoids metabolized in organs with the growth of soybean plant 大豆營養生長期種子中貯存的異黃酮逐步由種子向子葉,繼而向其它器官(主要是葉片)轉移,不同器官在植株生長發育過程中進行著異黃酮類物質的代謝。

Cotyledon and true leaves of stem mustard were cultured on ms medium supplemented with different concentrations of various cytokinins ( ba , cppu , tdz or kt ) alone or in combination with naa or iba , and the adventitious buds regeneration rate was evaluated . 以榨菜子葉、真葉為外植體進行離體培養,用ba 、 cppu 、 tdz和kt等細胞分裂素和生長素naa 、 iba組合誘導子葉、真葉再生不定芽試驗。

The average frequency of shoot regeneration in the cms line was 30 . 0 % and 2 . 34 % respectively and that in maintainer line was 30 . 0 % and 3 . 54 % respectively . on the other hand , explants of different sections of cotyledon resulted in different shoot regeneration abilities 不育系子葉與下胚軸的平均芽再生率分別為30 . 00和2 . 34 ;而保持系的平均芽再生率分別為30 . 0和3 . 54 。

Kt induced a slightly increase of cotyledon area in darkness and apparent enlargement was obtained under light especially wl , bl and rl . either light or kt could induced cotyledon enlargement and they showed independent and additive effect on the response 分析紅光和藍光與kt在促進子葉擴大中的作用,發現光與kt獨立地調節子葉擴大反應,二者主要表現為加性效應。

It is easy to induce callus derived from hypocotyls and cotyledons , but callus derived from root tip was rather difficult to be done . the most suitable medium for callus induction was the ms medium containing ba 0 . 5 mg / l and l . omg / l naa 愈傷組織的誘導與再分化的研究顯示: “種都一號”兒菜下胚軸與子葉愈傷組織誘導較容易,但根尖很難愈傷化。

2 establishment of genetic transformation system by agrobacterium rhizogenes the different types of explants ( hypocotyls , cotyledon , leaf blade , petiole ) were infected by strain r1601 of agrobacterium rhizogenes ( 2 0mg l )上培養30d后經過體細胞胚胎發生途徑產生大量的叢生苗;子葉培養在誘導子葉產生叢生苗培養基( msoc ) ms ba ( 2 0 。

2 . the sensitivity to so2 differs in various plant growth period . the resistant ability to so2 in cotyledon period > real leaves period . the ability in two real leaves and four real leaves have n ' t clearly difference 植物不同生育時期對so _ 2的敏感性不同,植物對so _ 2的抗性是子葉期真葉期, 2片真葉期和4片真葉期對so _ 2的抗性差異不顯著。

3 . through the ladder experiment of selecting transformer concentration by hyg , the proper selecting transformer concentration is 20mg / l hyg to tomato cotyledons and 35mg / l hyg to tobacco discs 經潮霉素梯度篩選試驗,最終確定番茄的適宜篩選濃度為20mg l ,煙草的適宜篩選濃度為35mg l ; 4

By the analysis of mutant growth we can conclude that the photoreceptors of phya , phyb and cryl are responsible for the cotyledon enlargement under fr , rl and bl , respectively Kt促進離體和連體子葉的擴大,黑暗條件下促進作用弱。在白光、紅光、藍光下kt對子葉的擴大效應明顯。

The explants of for root , hypocotyl and cotyledon were cultured . among them the hypocotyls from 20d or 30d seedings was the best for callus forming and anthocyanin production 以20d或30d齡的無菌苗下胚軸作外植體,效果好,愈傷組織生長量大,花色素苷的含量也高。