C - colicin 中文 - EN166

colicin n.【生物化學】大腸桿菌素。

colicky
In the 1990s , the pheromones of gram - positive bacteria , which regulates the growth and toxin secretion of the same type bacteria , were identified they were peptides consisted by dozens of amino acids . the pheromones can auto - recognize membrane receptor of the identical types of bacteria . we had constructed a fusion protein named pheromonicin by introducing a staphylococcal pheromone agrd at the c - terminal of the colicin ia 丘小慶等利用信息素的自主導向特性和大腸菌素ia的致死性通道特性構建了由金黃色葡萄球菌信息素agrd和大腸菌素ia組成的融合蛋白，命名為pheromonicin ，該蛋白表現出了信息素和大腸菌素ia都不具有的抗金黃色葡萄球菌活性。
The fusion protein was bactericidal active against staphylococcus aureus . in present study , we will truncate the none channel - forming do main , then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus . such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation 利用點突變方法將金黃色葡萄球菌信息素agrd ( i型， ystcdfim )的基因引入到大腸菌素fa梭基端1626基因上，并將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。
However , the receptor and translocation domains of the colicin ia can only recognize the inner and outer membrane receptors of the escherichia coli , therefore the wild type colicin ia can not be developed into antibiotics against other types of bacteria 然而由于其信號識別結構域只能識別大腸桿菌外膜和內膜上的特異受體，因此野生型大腸菌素難以拓展成為對抗它種細菌的抗菌素。
Colicin ia belongs to the family of bactericidal proteins , the channel - forming domain of which can form lethal ion channel in the membrane of escherichia coli and related strains such as shigella sonnei 大腸菌素ia是可形成離子通道的一種細菌素，其通道結構域( d451 ? i626 )能在同種異株的大腸桿菌內膜上形成致死性離子通道。