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chorioallantois n.絨(毛)膜尿囊。adj.-lantoic

chorioid

In this study , iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois . a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ) . the product of pcr was linked with suitable plasmid . then , the recombined plasmid was converted to escherichia coli . the converted escherichia coli 根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物,以增殖的兩株iltv的dna為模板,分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上,轉化感受態大腸桿菌,通過篩選對iltvtk基因的陽性克隆進行擴增培養。