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cercaria n.(pl. -riae )【動物;動物學】搖尾幼蟲,尾...

cercis

The target gene was then subcloned into plasmid vector and induced by iptg for its expression . after that , the recombinant protein was purified and used for the identification and characterization of its immunogenicity . the effect of the induced specific ige antibody on the hepatic granuloma formation and fibrosis after challenge infection with schistosome cercariae was evaluated Niptg誘導表達,產物沉淀中于約45kda處顯見高合蛋白表達帶, westernblot分析表明,該蛋白帶可被篩庫血清中特異性lge抗體識別;而載體本身表達的26gst蛋白帶則否。

Yherefore , many researchers have shown great concern with the development of vaccines to against schistosomiasis . at early stage , the research of vaccines of schitosomiasis was centered on dead vaccine and athenuated cercaria of schistosomes while molecular vaccine and athenuated cercaria of schistosomes while molecular vaccine is currently the focus of research with anti - infection protective immunity as its main concern . guan xiaohong and zhao weixian ( 1986 ) certified that the allergen of egg granulonma of schistosoma japonicum might firstly come from gut associated antigen ( gaa ) of schistosomula and adult worm and that daa had cross reaction with soluble egg antigen ( sea ) and membrane associated antigen ( maa ) ; and the gaa of schistosoma japonicum might play a sensitizing role in egg granuloma formation Np30主動免疫c57bl 6對尾蚴攻擊感染產生42 . 05的保護力,肝組織減卵率為66 . 63 ; balb c和昆明種小鼠的保護率分別為39 . 53和50 . 46 ;免疫山羊可誘導42 . 78的減蟲率,肝組織減卵率為35 . 83 ,糞減卵率為25 ,并可明顯抑制肝臟蟲卵肉芽腫的大小,肉芽腫數量明顯減少,纖維化減輕,體重明顯增加,因此np30是南京醫科大學博士學位論文很有希望的抗日本血吸蟲病疫苗侯選分子。

The mouse model of hepatic egg granuloma of schistosoma japonicum confirmed that np30 has sensitizing effect on formation of hepatic egg granuloma , active immunity in sheeps and mice with np30 can induce good protecti on against infection with schistosoma cercariae and reduces immunopathologic damage to host , np30 plays a significant down - modulatory role to hepatic granuloma and fibrosis 以往的研究業已證明np30對蟲卯肉芽腫的形成具有致敏作用,并有十分顯著抗蟲卵肉芽腫肝纖維化作用,用np30主動免疫小鼠、山羊可減輕日本血吸蟲對宿主造成的免疫病理損害。

Researches of schistosomiasis vaccines have gone more than 60 years , approximately including from the stages of dead vaccine and live vaccine ( irradiated attenuated cercariae vaccine ) to gene engineered vaccine , etc . many different forms of vaccines have been tested in animal models , including gluthathione s - transferase , paramyosin , irv - 5 , triose phosphate isomerase , sm23 , fatty acid binding protein ; which were considered promising by who / tdr . but none of them steadily accomplished the pre - set target level of 40 % protection . in order to enhance the protective capacity further , it is essential to develop novel vaccine antigens and / or vaccine adjuvants 血吸蟲病疫苗研究已有60多年的歷史,大致經歷了死疫苗、活疫苗(照射致弱尾蚴疫苗)和基因工程疫苗等研究階段,產生了一些who / tdr推薦認為很有希望的疫苗候選分子,如谷胱甘肽- s -轉移酶( gst ) 、副肌球蛋白( sm97 ) 、照射致弱疫苗抗原5 ( irv - 5 ) 、磷酸丙糖異構酶( tpi ) 、曼氏血吸蟲膜內在蛋白( sm23 )和脂肪酸結合蛋白( fabp , sm14 )等,但其對宿主的保護作用均不甚理想,未能穩定地達到40或以上的保護力水平,因此有必要繼續尋找新的疫苗抗原分子和/或疫苗佐劑,進一步提高其保護力。

Cercariae were collected , cultured in vitro and transformed to schistosomula in the rpmi 1640 medium with rabbit serum . the schistosomula were cultured in conditional media up to 96 hours . the number of schistosomula was counted and the death rate was calculated 將條件培養基與童蟲共培養, 96h內連續觀察計數,計算童蟲死亡率,與陰性對照比較,童蟲死亡率最高的基因池進入下一輪篩選。

G / dose / mouse ) . eight weeks after final boosting , vaccinated and control mice were challenged percutaneously with 40 ? 1 s . japonicum cercariae ( chinese mainland strain ) by the cover glass method 單獨np3o組:僅以np3ozo以g鼠免疫小鼠,免疫時間、次數均與ca np30組相同。

Observation of effects of 11 kinds of traditional chinese herbal medicine to prevent the cercaria of schistosoma japonicum from entering cutis 試論開展突發公共衛生事件癥狀監測的必要性和困難

Comparative study on the characteristics of immune response induced by 97kda dna and uv - attenuated cercariae vaccines of schistosoma japonicum 疫苗與致弱尾蚴疫苗誘導免疫應答特征的比較研究