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centrifugation

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centrifugation n.離心(分離)。

n. 離心(分離)。 “analytical centrifugation“ 中文翻譯：   分析離心[ 用光學系統連續地監測試樣在離心場中行為過程的方法。這種方法主要用; 分析離心分離“band centrifugation“ 中文翻譯：   區帶離心“centrifugation potentials“ 中文翻譯：   離心勢“centrifugation process“ 中文翻譯：   離心濃縮法“counterflow centrifugation“ 中文翻譯：   逆流離心分離“cscl centrifugation“ 中文翻譯：   氯化銫離心法“differential centrifugation“ 中文翻譯：   差示離心; 差速離心分離（作用）; 微差離心分離“double centrifugation“ 中文翻譯：   兩次離心“equilibrium centrifugation“ 中文翻譯：   平衡離心“fractional centrifugation“ 中文翻譯：   分部離心分離法; 分級離心; 分離心“gas centrifugation“ 中文翻譯：   氣體離心“gradient centrifugation“ 中文翻譯：   梯度離心分離法“gradient-centrifugation“ 中文翻譯：   梯度離心“interface centrifugation“ 中文翻譯：   界面離心分離“isodensity centrifugation“ 中文翻譯：   等密度離心,密度梯度離心; 密度梯度離心“isopycnic centrifugation“ 中文翻譯：   等比重離心; 等密度離心“nonequilibrium centrifugation“ 中文翻譯：   非平衡離心“preparative centrifugation“ 中文翻譯：   制備性離心,制備離心“refrigerated centrifugation“ 中文翻譯：   冷凍離心法“ultra centrifugation“ 中文翻譯：   超離心“zonal centrifugation“ 中文翻譯：   區帶離心法; 區帶離心分離“zone centrifugation“ 中文翻譯：   區帶離心“albumin gradient centrifugation“ 中文翻譯：   白蛋白梯度離心“aqueous phase separator centrifugation“ 中文翻譯：   水相分離器離心“centrifugation onto cell culture“ 中文翻譯：   細胞離心培養“centrifugate roll“ 中文翻譯：   離心橫滾

centrifuge

Cell morphology was similar no matter what method was used . percentage of live cells was significantly higher in erythrocyte splitting method that was ( 91 4 ) % than in centrifugation method that was ( 83 5 ) % . proliferative ability of mscs seperated by erythrocyte splitting method at 2 to 6 generation was much higher than by another method . the maximal cell number was 4 . 67 vs 4 . 10 times that of the initial cell number at cell inoculation 紅細胞裂解法較密度梯度離心法細胞貼壁較慢， 36小時才可見零星細胞貼壁，兩種方法細胞形態相似。活細胞百分比，紅細胞裂解法為（ gi士4 ） ，密度梯度離心治（ 83士5 ） ，有明顯差異。傳代培養： 2 6代范圍內，紅細胞裂解法較梯度離心法細胞增殖更旺盛（分別是按種時細胞數的4

After centrifugation at 8000rpm for 15 minutes , the bacterial pellets was subjected to ultr asonication in the suspension solution ( 50mmol / l , ph8 . 0 tris - hci , 100mmol / l nac1 , 0 . 5mmol / l edta , 4mol / l urea , 0 . 2 % triton x - 100 , 0 . 2 % doc ) at 300w , working 3s , intervul 3s for 240 times . then , concentration at 8000rpm for 15 minutes at 4 : 3 ) p (正x一們’一1一nk4表達菌株blzi誘導持續時間的研究分別進行了1 、 2 、 3 、 4 、 5小時的誘導，發現在誘導2小時后表山西醫科大學2003屆碩士學位論文達即可達到最人。

In the second trial , this modified discontinuous percoll gradient centrifugation method was introduced to isolate spermatids from the semen of fifteen male infertile patients . then the effect was identified by wright - giemsa stain , flow cytometry analysis , immunocytochemistry and fluorescence in situ hybridization ( fish ) . similary , the 22 % percoll fraction contained mostly haploid cells [ ( 91 . 85 ? 5 . 18 ) % ] ( p < 0 . 005 ) and the mean density in this fraction was ( 1 . 010 ? 0 . 786 ) x 105 / ml C法，對15例各種類型不育患者的精液細胞進行分離，并利用瑞姬染色法、流式細胞術、免疫細胞化學和熒光原位雜交oisffi等方法，從細胞形態特征、 dna倍體、細胞表面標i己與分化抗原，以及原位雜交信號的數目和位置結合細胞核特有的形態等方面加以鑒定。

In the first trial , combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis , and then a modified discontinuous percoll gradient centrifugation method ( 15 % , 22 % , 30 % , 40 % , 50 % , 60 % ) was introduced to isolate spermatids from the cellular suspensions . the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis , and the viability of spermatogenic cells was assessed by using eosin y exclusion test 在第一部分試驗中，首先利用連續3次組合酶消化成年小鼠睪丸制備睪丸細胞懸液，然后經6層非連續percoll梯度離心法( 15 、 22 、 30 、 40 、 50和60 )分離，通過形態學和流式細胞術鑒定南京醫科大學碩士學位論文各個percoll組分中精子細胞的含量，并以伊紅y排斥試驗測定細胞的存活率。

3 after incubated with 5 - bromo - 2 - deoxyuridine ( brdu ) for 7 days , newborn cells derived from regenerative neurospheres were immunostained . 4 the cells were collected by centrifugation separation and put into poly - 1 - lysine - coated terasaki wells , which were randomly divided into one of three groups of 24 wells cells each 4將傳代培養的神經球離心收集，轉移至24孔培養板，隨機分為3組，每組24孔細胞，置5 % coz 、 370c培養箱，連續培養7d ，觀察神經球的誘導分化。

A sicp reinforced al - cu alloy and al matrix composites are fabricated by a new method - electromagnetic centrifugal casting , the effects of rotational rate for centrifugation , magnetic density , particle size and matrix on the distribution of particles and microstructure of matrix are studied 首次利用電磁離心凝固方法制備sic _ p al基和sic _ p al - cu基復合材料，研究了離心轉速、顆粒尺寸、磁場和基體對顆粒分布以及對復合材料基體組織的影響。

In high speed centrifugation condition , the centrifugation temperature and nacl ionic intension of reverse extraction solution were changed to study their effects on the separation degree and the interaction of protein and aot , and the optimum centrifugation temperature and nacl addition were selected 摘要研究了在高速離心作用下，反萃液的離心溫度和鹽離子強度對aot和蛋白質的分離程度及相互作用的影響，并確定最佳離心溫度和鹽離子強度。

Report accidents and incidents promptly to the responsible person . these include spillage of infectious materials and chemicals , breakage of tubes during centrifugation , cuts , burns and contamination of non - intact skin or mucous membrane by potentially hazardous materials 發生意外或事故后須盡快向負責人員報告，包括傳染物料及化學品濺溢、離心沉淀時試管破裂，以及破損皮膚或黏膜被可能具危險性的物料割傷、燒傷或沾染。

This review summarizes the progression in preparation and preprocessing technologies of biological specimens . it especially introduces the preprocessing technologies , including centrifugation , filtration , dielectrophoresis , immunomagnetic separation , nucleic acid extraction 摘要介紹生物標本的制備與預處理技術的進展，其中，重點介紹生物標本的預處理技術，包括離心分離、過濾、兩相電泳、免疫磁性分離及核酸抽提方法。

The method for determining avermectin ( avm ) in muscle of sturgeon ( a . schrenckif ) by high performance liquid chromatography with fluorescence detector was established . the homogenized muscle sample was extracted by acetonitrile , following centrifugation at 3000 g for 15 min 采用乙腈作為肌肉中蛋白沉淀劑和阿維菌素的提取劑，經堿性氧化鋁spe柱純化，蒸干和衍生化，用高效液相色譜( hplc )檢測。

Two kind of ordered macroporous polymethylmetacrylate structures with different pore morphologies were fabricated from colloidal templates of the same silica microspheres , which was assembled by using centrifugation as driving force 摘要以離心沉降技術組裝的二氧化硅膠體晶體為模板，從同種尺寸的二氧化硅微球出發，合成了孔的結構和形貌不同的兩種有序大孔聚甲基丙烯酸甲酯。

The fetal liver stem cells were isolated by collagenase digestion , gravity sedimentation and density gradient centrifugation , identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition 采用膠原酶消化、重力沉降及密度梯度離心方法分離人胎肝干細胞，通過免疫細胞化學方法對其進行初步鑒定，以及應用流式細胞儀等對其生長狀況進行評估。

Studying on the technics of extraction and purification of herb dosage : extraction and purification , enzyme reaction . super fluid extraction , strong immersion , new type of absorption and defecation , super speed centrifugation and film separation , and dissolve leach technique 3研究與開發任務1植物化學有效成分標準品和對照品的研究開發，中藥指紋圖普庫與資源庫的建立

Results obtained included : ( 1 ) mscs began to attach to culture flask after 24h cell inoculation by centrifugation method . cell proliferation was active after 3d . and mscs began to attach to culture flask only after 36h cell inoculation by erythrocyte splitting method 研究結果顯示： （ 1 ）密度梯度高心法接種后24小時可見少數細胞貼壁， 3天后，細胞增殖活躍。

The enriched stable isotope tracer technique combined with neutron activation analysis and differential centrifugation was utilized to investigate the subcellular distribution of rare earth elements sm and yb in rat liver Sm和, sup 168 yb作為示蹤劑，結合差速離心分離和中子活化分析技術naa初步研究了稀土元素sm和yb在大鼠肝臟亞細胞組分中的分布。

Methods : gradient centrifugation and differential adherence were employed to isolate type pneumocytes from the lungs in the rats , proliferative activities were compared in the cultued cells from the young , adult and aged rats 方法：應用梯度離心和貼壁培養分離大鼠肺泡型細胞，比較幼年、中年和老年大鼠培養肺泡型細胞的增殖能力。

Cells were then harvested by centrifugation and the pellet was resuspended in phosphate - buffered saline ( pbs ) containing 5 mm edta . after sonication , debris was removed by centrifugation at 10000 x g for 15 min at 4 挑取轉化后的大腸桿菌提取質粒， ecori和hindln酶切質粒進行鑒定，瓊脂搪電泳顯示含有大約800kb的目的片段。

The culture supernatants of heat - shocked and control a20 cells were collected to isolate exosomes through a sequence of differential centrifugation steps . briefly , cells were removed by centrifugation for 5 min at 300x gmax 在透射電鏡下hs一exo形態與exo類似，呈50一sonm的雙層膜囊泡樣結構，大小比較均一，結構完整。

Then bacteria were collected by centrifugation and split by ultrasonic method . the purified products were analyzed by tris - tricine sds - page . the immuno - activity of the recombinant proteins were analyzed by western blot 將離心收集到的菌體超聲破壁，離心分離，收集上清液，進行離子交換層析和分子篩分離純化。