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carbenicillin n.【藥學】羧芐青霉素,卡比西林。

carbhemoglobin

The results also revealed that it was a reasonable choice to use carbenicillin as the antibiotics of inhibiting growth of remnant agrobacterium after co - culture , and when hygromycin was used as the selective agent , continuous selection at low concentrations ( 50 ~ 150mg / l ) produced the highest numbers of transgenic plants without escapes 試驗表明,在農桿菌介導高羊茅遺傳轉化中,抑菌劑宜選用梭節青霉素;以潮霉素作為選擇劑時,采用低濃度( 50一15om留l )連續篩選的方式比較合適,在該方式下,獲得的轉基因植株較多。

Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and loomg / l kanamycin and incubated at 25 , 16 / 8h light / dark cycle . small leaves of adventitious shoots differentiated from explants were cut and dipped into gus staining solution . positive shoots , gus tinted , were induced to root 分化出的不定芽切下半張葉片進行gus染色,呈陽性的植株從外植體上切下,在生根培養基( ms 0刀sing lnaa 50mg幾kan )中誘導根的形成。

Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and 150mg / l kanamycin and incubated at 25 , 16 / 8h light / dark cycle . leaves of adventitious shoots were detected by gus staining Sin旮16ea )上暗培養3天后,移入加有抗生素150mg ikan和500mg幾cr的誘芽培養基上誘導不定芽的分化。