bobwhite n.【鳥類】北美鶉。
n. 【鳥類】北美鶉。 “crested bobwhite“ 中文翻譯： 冠齒鶉“black-throated bobwhite“ 中文翻譯： 黑喉齒鶉“bobwig“ 中文翻譯： 短而拳曲的假發“bobweight feel system“ 中文翻譯： 配重式載荷感覺裝置“bobwire“ 中文翻譯： 刺鐵絲“bobweight balancing bungee“ 中文翻譯： 配重平衡彈簧“boby“ 中文翻譯： 寶貝數碼; 博比; 慕容小草“bobweight“ 中文翻譯： 配重; 平衡錘; 平衡重“boby bonds“ 中文翻譯： 蓄債券“bobvos“ 中文翻譯： 博布沃什“boby carrier“ 中文翻譯： 嬰兒包,童車“bobutac“ 中文翻譯： 博布察克
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In the experiment , the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked . it was expressed in e . coli and its protein was determined . after having been properly modified , the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing , which is the prerequisite work for genetic transformation 本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，并在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。 |
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In our experiment , the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ) , then sequenced . the cloned sequence was completely identical to the sequence which was issued in genbank 本實驗采用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz ( + ) ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，并且序列與genbank中發表的序列完全一致。 |