B - blastula 中文 - EN166

blastula n.(pl. -lae ) 【生物學】囊胚。

blat
G - banding the gtg banding ( g - banding ) was carried out by the standard trypsin method with slight modification , which works well for protochordate because a good number of reproducible g - bands are consistently obtained from the embryonic cells of late blastulae and early gastrulae of amphioxus b . belcheri tsingtauense G帶型用稍作修改的標準的胰酶顯帶技術，進行gtg帶紋的顯示，它們能較好地顯示頭索動物青島文昌魚的晚期囊胚和早期原腸胚的中期染色體的g帶，并且重復性好。
Using m199 containing 20 % calf bovine serum and 11 % dmso as the diluent and by the methods using in cryopreservation of embryo cells of misgurnus auguillicaudatus , two groups of cells derived from blastula of grass carp were preserved in liquid nitrogen at - 196 . after 6 days cryopreservation , one group of cells were thrawed and the percentage of viable cell was about 72 % ; the other , cryopreserved for 13 days , was 52 以細胞培養液m199 (含20 %的小牛血清)為稀釋液， dmso的濃度為11 % ;與泥鰍胚胎細胞冷凍保存方法一樣，采取先慢后快的方式，冷凍保存兩組草魚囊胚晚期細胞于一1 %的液氮中。第一組冷凍保存6天后解凍，成活率為72 % ，第二組冷凍保存13天后解凍，成活率為520 / 0 。
The primary results showed : using m199 as diluents containing 20 % bovine serum , it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example , from 0 to - 6 freezing speed is about - 0 . 05 a minute , from - 6 to - 40 , freezing speed is about - 0 . 5 a minute ) , studies on effect of various concentration of dmso demonstrate that about 12 . 5 % dmso gave the highest post - thaw percentage of viable cells . the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus . the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula . the cells preserved in liquid nitrogen at - 196 were thawed and cultivated , a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 % . the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells 研究初步表明:以細胞培養液m199 (含2既的小牛血清，常規量雙抗)為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜采取先慢后快的方式(例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ) ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天后均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。
The fertilized egg entered the 4 - cell stage in 2hlomin , and the 8 - cell stage in 2h20min . the fertilized egg entered the 32 - cell stage in 3hl2min , and the cells distributed in different layers . the fertilized egg entered the multi - cell stage in 4h05min and the early blastula stage in 5h 1小時30分受精卵開始第一次分裂； 2小時10分進入4胞期； 2小時20分進入8胞期： 3小時12分進入32胞期，細胞出現分層現象； 4小時05分進入多細胞期； 5小時進入高囊胚期； 6小時50分進入低囊胚期。
Purpose embryonic stem cells ( escs ) which are from the inner cell mass ( icm ) of preimplantation blastula are pluripotent . neurons are considered as terminally differentiated cells that can not divide nor proliferate after injury 目的胚胎干細胞( embryonicstemcells ， escs )來源于著床前胚胎的囊胚期內細胞團( innercellmass ， icm ) ，具有發育全能性。