B - basta 中文 - EN166

basta int.〔意大利語〕夠了!罷了!足了!

bastard
The house was pretty full . besides sir malcolm and his two daughters , there were seven more people , a scotch couple , again with two daughters ; a young italian contessa , a widow ; a young georgian prince , and a youngish english clergyman who had had pneumonia and was being chaplain to sir alexander for his health s sake . the prince was penniless , good - looking , would make an excellent chauffeur , with the necessary impudence , and basta 別墅差不多住滿了容，除了麥爾具體地說爵士和他的兩個女兒外，還有七位客人：一對蘇格蘭夫婦，也帶了兩個女兒一位是年輕的意利的伯爵夫人，她是個寡婦一位是年輕的喬治亞親王另一位斷紀還勸的英國牧師，他因為患過炎，現在在亞力山大爵士的小教堂里主事，藉此休養身體。
By using primers designated for lacz gene , pcr result suggested an integration of lacz into u . pinnatifida genome . nine reagents including penicillin g , kanamycin , g418 , teicoplanin , zeocin , chloramphinicol , hygromycin , basta and methotrexate were tested as selective reagents for selection of transgenic sporophytes . the results showed that young sporophytes of u . pinnatifida were sensitive to chloramphinicol and hygromycin and very sensitive to basta which suggest the potential of using the resistance genes as selectable markers ，為了解決轉基因裙帶菜的篩選問題，進行了裙帶菜幼孢子體對不同篩選壓力的敏感性實驗，包括抗生素類的青霉素g 、卡那霉素、 g418 、硫酸鏈霉素、鹽酸潔霉素、 zeocin 、氯霉素、潮霉素，除草劑類的basta ,氨甲喋呤類的氨甲喋呤，結果顯示裙帶菜幼孢子體對氯霉素、潮霉素敏感，對basta非常敏感。
Obtaining transgenic male sterile tobacco in order to prove that hsp70 antisense cdna can lead to male sterility , with plasmid 3301 + 650 , 3301 + 651 we transformed 207 aspetic tobacco leaves by genegun bombarding and agrobacterium mediation ( 109 by genegun bombarding , 98 by agrobacterium ) . by cultivating them in blotting media containing basta 0 . 4 mg / 1 , we get 181 resistant leaves ( 98 by genegun bombarding , 88 by agrobacterium mediating ) 獲得轉基因雄性不育煙草為了證實hsp70反義cdna能創造雄性不育，我們將3301 + 650和3301 + 651質粒用基因槍和農桿菌介導法轉化煙草無菌發芽的葉片，共207片(基因槍109片，農桿菌98片) 。在含basta0 . 4mg l的篩選培養基上進行篩選，得到抗性葉片181片(基因槍93片，農桿菌88片) 。
A genetic transformation model for the brown seaweed undaria pinnatifida has been primarily set up by using micro - particle bombardment as the method , female or male gametophytes as the gene recipients , hybridization as the regeneration route and chloramphenicol , hygromycin or basta as selective reagents 本文從轉化受體、轉化方法、報告基因、再生途徑、篩選方法等方面對裙帶菜的遺傳轉化進行了研究。首先，分離并建立了裙帶菜雌雄配子體的無性繁殖系，進行了裙帶菜的不同再生途徑的研究。
The experiment was conducted in a 2 . 4 ha isolated field mimic rice production practice with pollen competition . a japonica gm rice l201 containing bar gene with herbicide basta resistance was used as a pollen donor and six indica hybrid rice varieties and its male sterile ( ms ) lines and two common wild rice ( oryza rufipogon and o . nivara ) that share same aa genome with cultivated rice were used as recipients 本試驗選擇廣州作為華南水稻生態區的代表，以含bar基因(抗除草劑basta )的轉基因粳稻l201為花粉供體，模擬大田生產實際，對轉基因向秈型兩系及三系雜交稻不育系、雜交稻品種及含aa基因組的普通野生稻的基因漂流及其影響因素進行了研究。
( 2 ) gene flow frequency was reduced as distance from pollen donor increased and a dramatic reduction occurred at about 2 meters . the maximum distance where gene flow was not detected was 50 m for hybrid rice while it was 70 m for ms lines , with an exception that in one of the four ms lines it was detected a frequency of gene flow 2 . 8 + 10 - 6 at 150 m for zhong 9a 在開花期主流風向ne的風速為0 . 2 ? 2 . 2m / sec的條件下， 2個雜交稻品種的最大漂流距離為40m ； 4個不育系的基因漂流基本上到60m為止， 70m處基因漂流頻率均降為0 ，僅中9a在150m處發現了1株basta抗性苗，經pcr檢測驗證為陽性。
These confirmed the successful transformation of the a78 - 3 and a78 - 4 on selection medium containing basta . microarray on membranes were fabricated from a set of 384 pinus taeda genes ( cdnas ) related to lignin synthesis , adaptation or primary metabolism for examination of gene expression in the sublines . the results showed : ( 1 ) the correlation coefficients between the transgenic sublines a78 - 3 and a78 - 4 and the untransformed control a95 : 88 : 22 were 0 . 8028 - 0 . 9028 , while those of a78 - 5 are 0 . 8897 - 0 . 9302 選擇384個與木質素生物合成及植物生理代謝和環境適應性有關的基因或cdna片段構建尼龍芯片膜，并對轉基因細胞亞系a78 - 3 、 a78 - 4和a78 - 5和對照亞系a95 ： 88 ： 22等培養再生植株進行基因表達的微陣列檢測，結果表明： ( 1 )三個亞系與對照之間的pearson相關系數分別為0 . 8607 、 0 . 7975和0 . 9630 。
Because the phenotype of osdd2 mutant was segregated with the basta resistance , it suggested that the mutation was arose by disruption of the wrky gene 根據已有的共分離數據和初步分析結果表明osdd2突變體的發育遲緩性狀是有外源基因插入引起。
Ppt ( phosphinothricin ) is the active composition of broad - spectrum , non - selective hercicide basta 廣譜、非選擇性除草劑basta的活性成分是草丁膦( phosphinothricin ) 。
Fiorentina and lazio are keeping tabs on red star belgrade midfielder dusan basta 拉齊奧和佛羅倫薩一直在密切注意著貝爾格萊德紅星隊的巴斯塔