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antiserum

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antiserum n.【醫學】抗血清。

n. 【醫學】抗血清。 “absorbed antiserum“ 中文翻譯：   吸收抗血清“adenovirus antiserum“ 中文翻譯：   抗腺病毒血清; 腺病毒抗血清; 腺簿抗血清“adsorbed antiserum“ 中文翻譯：   吸附的抗血清“antianmion antiserum“ 中文翻譯：   抗羊膜抗血清“antiserum agar“ 中文翻譯：   抗血清瓊脂“antiserum ananaphylaxis“ 中文翻譯：   被動抗過敏性“antiserum anaphylaxis“ 中文翻譯：   抗血清過敏性; 抗血清性過敏癥“antiserum anthracis“ 中文翻譯：   精制抗炭疽血清“antiserum antistreptolysin“ 中文翻譯：   抗鏈球菌溶血素抗血清“antiserum asnanaphylaxis“ 中文翻譯：   被動抗過敏性“antiserum challenge“ 中文翻譯：   抗血清檢驗“antiserum effects“ 中文翻譯：   抗血清效應“antiserum globulin“ 中文翻譯：   抗血清球蛋白“antiserum production“ 中文翻譯：   抗血清產量; 抗血清形成“antiserum test“ 中文翻譯：   抗血清試驗“antiserum to somatostatin“ 中文翻譯：   生長激素釋放抑制因子抗血清“botulinum antiserum“ 中文翻譯：   肉毒抗毒素,抗肉毒血清“coxsackievirus antiserum“ 中文翻譯：   柯薩奇病毒抗血清“defatted antiserum“ 中文翻譯：   脫脂抗血清“diphtheria antiserum“ 中文翻譯：   抗白喉血清“drug antiserum“ 中文翻譯：   藥物抗血清“enterovirus antiserum“ 中文翻譯：   腸病毒抗血清; 腸道簿抗血清“heterologous antiserum“ 中文翻譯：   異種抗血清“homologous antiserum“ 中文翻譯：   同種抗血清“antiserum adenoviri purificatum“ 中文翻譯：   精制抗腺病毒血清; 精制抗腺簿血清“antiserotonin“ 中文翻譯：   抗-5-羥色胺; 抗5-羥色胺劑; 抗血清素

But the extensive cross - reactivity and the lacks of the ideal antisera make it difficult in technique and the false result . furthermore , the lack of the standard antisera also cause the “ blank “ result in a few newly discovered alleles 但血清學方法不僅存在著廣泛的交叉反應和難以找到理想的抗血清而出現技術上的困難和分型判斷誤差，還會有許多新發現的等位基因卻缺乏相應的標準抗血清，導致個別基因型無法檢出。

antiskid

The activity of the antiserum was tested by dot enzyme immunization assay ( diba ) with purified antigen . western blot of hela nuclear protein extract , which contained natural hbaf53 , showed that the antiserum is also a specific antiserum to natural hbaf53 . therefore the highly specific and sensitive antiserum can be applied to various studies 用純化的抗原蛋白，經斑點印跡試驗測得baf53抗血清的效價，又用提取的hela細胞核蛋白(含有天然baf53蛋白)進行免疫印跡分析( westernblotting ) ，證明天然baf53蛋白也是該抗血清的抗原，說明獲得的多抗血清具有高特異性和敏感性，可用于多方面的研究。

A piezoelectric immunosensor was developed by immobilized transferring antiserum on the surface of quartz crystal to especial answer transferring , it can be get good answer to determined in the range of 3 . 92 ~ 79mg / l ; the adsorption and desorption of consult serum on the quartz crystal monitored with tr - antibody , determined the rate constant of immuno - reaction was 2 . 14xl07 ( mol / l ) ' 1xs ' 1 , the association constant of the immuno - reaction ( k1ss ) was 6 . 75xl07 ( mol / l ) ~ l Oz vomg l范圍內的參考血請中的轉鐵蛋白進行了定量測定，獲得很好的響應；利用石英晶體微天平技術在線監測了參考血清在固定了轉鐵蛋白抗體的石英晶振上的吸附與解吸過程，測得了其抗原抗體免疫反應的速率常數為2 14xl0 ’ （ mol l ） ’ xs “ ‘ ，結合常數為6

The western blot analysis show that the antiserum induced by the new antigen plus hemocyanin could bind with the 22kd and 55kd proteins , which were existed in the testis tissue protein of mouse , rat and human . 2 ) the purified peptide emulsified in an equal volume of freund ' s adjuvant and immunized the female balb / c mice with 8 - 10 weeks old . the antiserums and the washings of vaginal membrane were detected by elisa , and shown the highest level of the specific antibody igg was 1 : 6000 , while the iga was 1 : 300 二、 p3多肽與弗氏佐劑混懸后免疫近交系balb c小鼠后，在血清和陰道粘膜沖洗液中可檢測出特異性lgg 、 lga ，最高效價分別達到1 ： 6000和1 ： 300 ；免疫后的小鼠脾臟淋巴細胞增殖率升高，淋巴細胞培養上清液中分泌的il 4和inf y也升高，且以il 4更明顯。

Addition of antiserum specific to cam to a whole cell extract reduced binding of the hsf to hse at 44 . re - addition of cam to the cell extract pretreated with antiserum might restore the ability of the hsf to bind to hse . dna - binding activity of the hsf was promoted by directly adding cam to a whole cell extract at 44 , but not by the same concentration of s100 and bsa 兔抗cam血清也明顯抑制44熱激誘導的hsf的dna結合活性，而回加cam又可恢復其活性；熱激條件下直接以cam處理可促進hsf的dna結合活性，同樣濃度的s - 100和bsa無明顯作用；另外，在非熱激條件下外加cam還可替代熱激誘導hsf的dna結合活性，而同樣濃度的bsa處理無此作用。

All the clones could react with swine antisera against prrsv and mouse antisera to prrsv , suggesting aa50 ~ aa55 domain on n protein might be the antigenic site recognized by mab ge3 . 4 . the gene fragment encoding the epitope recognized by mab ge3 was cloned into the expression vector pgex - 4t - 2 用prrsv陽性血清和鼠源抗prrsv抗體采用間接elisa進行鑒定，結果表明篩選到的噬菌體克隆可以與血清發生特異性反應，從而初步確定了單克隆抗體ge3所識別的抗原表位。

3 . preparation of polyclonal antiserum against angiotensinogen in order to study agt in protein level and also for other continuing work , the c - teminus of rat angiotensinogen was expressed in e . coll . rabbits were immunized by this expressed 6his - agtc protein and serum from different rabbits were raised 血管緊張素原多克隆抗血清的制備為研究agt蛋白表達及后續工作，在大腸稈菌中融合表達了agt蛋白的c末端，以此融合蛋白為抗原免疫家兔，制備抗血清。

Its content was about 9 . 8 % among total cell protein by gene genius bio imaging system . the fusion proteins were found largely in an insoluble inclusion bodies . the purified fusion proteins was obtained by his6 technique used to immunize rabbits to obtain polyclonal antiserum with titer of 1 * 105 經工ptg誘導，重組質粒在點co力‘ blzi中表達出了c端融合了6xhis的融合蛋白，過量表達的蛋白主要以不溶性蛋白形式存在，其表達量占菌體總蛋白的9 . 8 % 。

The molecular approach to detecting peptide hormones using cdna probes should also be much faster than the immunological method because it can take years of tedious purifications to isolate peptide hormones and then develop antiserums to them 采用cdna探子來測定肽激素的這一分子生物學方法同時也應該比免疫學的方法速度來得快，因為對于免疫的方法來說，需耗費好幾年枯燥的提純進程，方能將肽素分離了出來，然后再培養出針對它們的抗血清。

But the extensive cross - reactivity and the lacks of the ideal antisera make it difficult in technique and the false result . furthermore , the lack of the standard antisera also cause the “ blank “ result in a few newly discovered alleles 但血清學方法不僅存在著廣泛的交叉反應和難以找到理想的抗血清而出現技術上的困難和分型判斷誤差，還會有許多新發現的等位基因卻缺乏相應的標準抗血清，導致個別基因型無法檢出。

Research on envelope protein - based hcv vaccine demands a prompt solution to the problems of detection of antigen and evaluation of post - vaccination immune responses , which requires large amounts of highly purified envelope proteins and corresponding antibody or antiserum 基于被膜蛋白的hcv疫苗研究急需解決抗原檢測和疫苗接種后的免疫反應評價的問題，需要首先得到大量高純度的被膜蛋白抗原及其相應抗體或抗血清。

In this study , we at first aimed at obtaining the gene encoding the specific ige antibody related proteins by immunoscreening the schistosoma juponi cum adult worm cdna library with the pooled high - titer ige antisera from the individuals living in the schistosome epidemic regions J測定。 jnij序結果顯示，該插入片段為1200hp ，第一開讀框長507hp ，編碼169個氨基酸殘基，理論分子量為19 3kdi 。

This confirmed that the fr - 008 pks gene in the e . coli expression vectors was cloned in the correct reading frame . the above detection of a very large protein in s . sp . fr - 008 indicated that the antiserum had the desired specificity for the fr - 008 pks 這說明構建的大腸桿菌表達質粒中fr - 008pks基因是以正確的讀碼框架克隆和表達的；同時也表明抗血清對天然的fr - 008型pks具有特異性。

Using agarose gel double diffusion method , the antiserum of pea ferritin can cross - react with the crude extraction of soybean seed ferritin . the result indicated that high specific antiserum had been acquired and could be used ~ in the detection of the expressi 5 、植物鐵蛋白的免疫學檢測瓊脂糖免疫雙相擴散法顯示，制備的豌豆鐵蛋白抗血清可與大豆鐵蛋白粗提物發生免疫交叉反應。

Analysis of h9 subtype : ( 1 ) seven out of fourteen h9 strains tested shared similar reaction patterns with dk / hk / y280 / 97 or ck / hk / g9 / 97 virus , while the rest showed almost no inhibition and low reactivity to all reference antisera Hg亞型的分析： （ 1 ）抗原性分析表明七株hgnz病毒與w hk yzso 97和n hk gg 97病毒反應相似。而其它病毒株與抗血清不反應或反應的滴度很低。

Following the purification of the expressed protein , a elisa was developed to detect reference antisera of h7 , h5 and h9 subtypes . the result indicated that h7 antisera was positive , h5 and h9 antisera were negative 用電洗脫方法純化菌體表達ha1蛋白后，建立了間接elisa方法，并對aivh7 、 h9 、 h5亞型血清進行檢測，結果h7亞型血清呈陽性， h5 、 h9亞型血清呈陰性反應。

In chapter 4 , renewable arnperometric immunosensor based on sol - gel technique has been developed by dispersing graphite , complement 3 antiserum and sol - gel at low temperature for the determination of complement 3 in human serum 這些研究對于新型抗癌藥物的設計和腫瘤的化學治療有指導意義，同時，對于建立簡便而有效的抗癌藥物篩選方法也良有幫助的。

The specific band was excised from the gel and injected into mice . the antiserum was collected from the immunized mice and used for indirect fluorescent assay ( ifa ) with chicken embryonic firoblast ( cef ) infected by mdv 誘導菌體的裂解物經12的sds聚丙烯凝膠電泳( sds - page )和western - blot試驗驗證，得到大小為43kd的融合蛋白，與預期大小一致。

Immunohistochemistry and western blotting further confirmed that the prepared antiserum could recognize not only the prokaryotic expressed 6his - agtc , but also the endogenous agt protein in many tissues of rat and human Western印跡分析及免疫組化顯示，所制備的抗血清不僅可識別原核表達的agt片段，而且能夠識別大鼠及人多種組織中內源性agt蛋白。