x
uploads/cytokinesis.jpg

cytokinesis n.【生物學】細胞質分裂,減數分裂(=cytodiere...

cytokinin

By using gfp - cam fusion protein , we have observed the detailed dynamic redistribution of cam in hela cells during cytokinesis . in tri - polar cell model , we have studied the relationship between the distribution pattern of cam and the formation of the cleavage furrow . furthermore , we have made the study on the regulation mechanism of cam during the whole process of cytokinesis by inhibiting its activity at different cytokinesis stages 我們采用綠色熒光蛋白( gfp )標記技術,重點觀察了胞質分裂期hela細胞中gfp - cam的動態分布;應用三極細胞模型研究了cam的分布與分裂溝形成之間的關系;還通過在胞質分裂不同階段抑制cam的活性研究了cam在整個胞質分裂過程中的作用機制。

3 . when the cells were treated with w7 , a ca2 + / cam inhibitor , the central spindle assembly was affected , and the abnormal central spindle was formed ; besides , when w7 treatment was applied at an early stage of cytokinesis , the division furrow index would decrease substantially . these results show that cam is really involved in the regulation of furrow formation and this involvement may be fulfilled by regulating the dynamic structure of central spindle 我們利用w7抑制cam活性,發現cam活性喪失會引起中區紡錘體結構異常;如用w7處理同步化于胞質分裂早期的細胞,可使細胞的分裂溝形成指數明顯下降,這表明cam的確參與調控了分裂溝的形成,而且可能是通過調控中區紡錘體的動態結構參與這一過程的。

In this paper we reported the engagement of cortactin in cell movements in vivo for the first time . we also found that , besides the traditional idea about the role it plays in cell motility , cortactin was also involved in cytokinesis and played undispensensable role in nleava stage independent of arp2 3 complex 這表明皮層蛋白除了傳統意義上了解的參與細胞主動運動外,還在卵裂期發育事二且j中科院碩士學位論文喻丹皮層蛋白( cortactin )在斑馬魚早期胚胎發育中的分布圖式和功能分析件中扮演重要角色。

We also stained the embryos with anti - arp3 antibody and found that these two kinds of antigen are generally co - localized but arp3 were not detected in cleavage stage embryos . it was deduced that cortactin functions in the cytokinesis independent of arp3 . and microinjection of anti - cortactin antibodies into the i cell stage embryos leaded to fatal developmental defects because of the unequal cytokinesis 在利用抗ap3的抗體進行的胚胎整裝染色中,激光掃描共聚焦顯微鏡觀察發現, arp3在卵裂期沒有表達, mbt后,在細胞皮層區檢測到arp3蛋白的陽性染色,在隨后的發育過程中arp3與皮層蛋白共分布。

W7 was added when the cells entered anaphase , we found that although some cells had elongated , the distribution patternof cam at central spindle was normal , and it seemed that a cleavage furrow would possibly form soon , however , these cells stopped cytokinesis at this stage , no furrow ingression followed 在細胞進入有絲分裂后期時,加入w7 ,我們發現有的細胞雖已拉伸變長,中區紡錘體處也有gfp cam的分布,存在形成分裂溝的跡象,但分裂溝卻不繼續縊裂加深。

Homologues of era have been identified in prokaryotes and eukaryotes . there are two domains in era : the n - terminal gtp - binding domain and c - terminal rna binding kh domain . era plays an important role in cell cycle progression at a specific point in the cycle , after chromosome partitioning but before cytokinesis Era是在原核和真核生物以及植物中普遍存在的g蛋白,是一個結構上既具有gtp結合結構域又有rna結合結構域、不同于ras的獨特的新的g蛋白亞家族。

As for the sub - cellular locations of cortactin , laser confocal scanning microscope pictures shew that in cleavage stage , cortactin was distributed ubiquitously in the cytoplasm with the exception that in cells undertaking cytokinesis , positive stained signals were detected in the cleavage furrow 在利用激光掃描共聚焦顯微鏡進行的皮層蛋白亞細胞定位的觀察中發現,卵裂期,皮層蛋白在整個細胞質中分布,在正在進行胞質分裂的卵裂溝中染色較強。

When the cells synchronized at a late cytokinesis stage were treated with w7 , the dissolving of the midbody was delayed ; furthermore , we found that the dissociation of y - tubulin from the midbody was also inhibited W7處理同步化在胞質分裂后期的細胞,不但使中體解聚發生延遲,而且抑制了y一微管蛋白與中體的解離。

This dynamic colocolazation pattern of cam with different cytokinesis apparatuses indicates that cam involves in the whole process of cytokinesis . 2 Cam在胞質分裂期不同階段與不同特征結構的共分布提示cam參與了胞質分裂的整個過程。

By using the gfp - labeling technique , we have found that gfp - cam colocalized with all the characterized structures formed during cytokinesis 通過采用gfp標記技術,我們觀察到gfp - cam與胞質分裂期細胞內形成的特征結構存在共分布。

Cytokinesis the division of the cytoplasm after nuclear division ( mitosis or meiosis ) 胞質分裂:隨著核(有絲分裂和減數分裂)分裂之后的細胞質的分裂。

Cytokinesis block method 胞漿阻滯法

Septins and cytokinesis 隔蛋白和胞質分裂

Cytokinesis block micronucleus method 胞漿阻滯微核法